Nathan Fillion
Malcolm 'Mal' Reynolds
Summer Glau
River Tam
Gina Torres
Zoë Alleyne Washburne
Alan Tudyk
Hoban 'Wash' Washburne
Morena Baccarin
Inara Serra
Adam Baldwin
Jayne Cobb
Jewel Staite
Kaywinnet Lee 'Kaylee' Frye
Sean Maher
Simon Tam
Ron Glass
Shepherd Derrial Book
Chiwetel Ejiofor
The Operative
David Krumholtz
Mr. Universe
Michael Hitchcock
Dr. Mathias
Sarah Paulson
Dr. Caron
Yan Feldman
Mingojerry 'Mingo' Rample
Rafael Feldman
Fantastic "Fanty" Rample
Nectar Rose
Lenore
Tamara Taylor
Teacher
Glenn Howerton
Lilac Young Tough
Hunter Ansley Wryn
Young River Tam
Logan O'Brien
Boy Student
Erik Erotas
Boy Student
Demetra Raven
Girl Student
Marley McClean
Girl Student
Jessica Huang
Girl Student
Scott Kinworthy
Ensign
Erik Weiner
Helmsman
Conor O'Brien
Lab Technician
Peter James Smith
Lab Technician
Weston Nathanson
Trade Agent
Carrie "CeCe" Cline
Young Female Intern
Chuck O'Neil
Vault Guard
Amy Wieczorek
Lilac Mom
Tristan Jarred
Lilac Son
Elaine Mani Lee
Fan Dancer
Terrence Hardy Jr.
Mining Camp Boy
Brian O'Hare
Alliance Pilot
Ryan Tasz
Black Room Soldier
Colin Patrick Lynch
Black Room Soldier
Terrell Tilford
News Anchor
Joshua Michael Kwiat
Slovenly Beaumonde Man
Antonio Rufino
Bartender (uncredited)
Linda Wang
Chinese Snake Dancer (uncredited)
Rick Williamson
Bar Guy (uncredited)
Neil Patrick Harris
Screaming Reaver (uncredited)
Matt McColm
Lab Security (uncredited)
Marcus Young
Bar Security (uncredited)
Dennis Keiffer
Bar Guy (uncredited)
Mark Winn
Futuristic Worker (uncredited)